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American Focus > Blog > Tech and Science > How E2B became essential to 88% of Fortune 100 companies and raised $21 million
Tech and Science

How E2B became essential to 88% of Fortune 100 companies and raised $21 million

Last updated: July 28, 2025 8:40 pm
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How E2B became essential to 88% of Fortune 100 companies and raised  million
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E2B, a startup specializing in cloud infrastructure for artificial intelligence agents, has successfully concluded a Series A funding round of $21 million led by Insight Partners. This funding round comes at a time when there is a significant surge in demand for AI automation tools within the enterprise sector.

The company has reported that an impressive 88% of Fortune 100 companies have already signed up to utilize E2B’s platform, showcasing the rapid adoption of AI agent technology within enterprises. The Series A funding round also saw participation from existing investors such as Decibel, Sunflower Capital, and Kaya, as well as prominent angels like Scott Johnston, former CEO of Docker.

E2B’s technology addresses a crucial infrastructure gap as companies deploy AI agents for various tasks like code generation, data analysis, and web browsing. Unlike traditional cloud computing designed for human users, E2B offers secure, isolated computing environments where AI agents can safely execute potentially risky code without compromising enterprise systems.

“Enterprises have high expectations for AI agents. However, we’re requiring them to scale and perform on legacy infrastructure not designed for autonomous agents,” said Vasek Mlejnsky, co-founder and CEO of E2B, in an exclusive interview with VentureBeat. “E2B addresses this by providing AI agents with secure, scalable, high-performance cloud infrastructure tailored for large-scale agent deployments.”


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Seven-figure monthly revenue spike shows enterprises betting big on AI automation

The recent funding round reflects a significant increase in revenue for E2B, with the company adding “seven figures” in new business in just the past month. E2B has processed hundreds of millions of sandbox sessions since October, demonstrating the scale at which enterprises are deploying AI agents.

E2B’s clientele includes leading AI innovators such as search engine Perplexity, AI chip company Groq, and workflow automation platform Lindy. These companies rely on E2B for various AI-related functionalities, showcasing the platform’s versatility and effectiveness.

Moreover, E2B’s technology has become integral to AI research efforts, with entities like Hugging Face and UC Berkeley’s LMArena leveraging E2B for their experiments and evaluations.

Firecracker microVMs solve the dangerous code problem plaguing AI development

E2B’s innovative use of Firecracker microVMs plays a crucial role in providing safe, isolated environments for AI-generated code execution. This approach addresses the security challenges associated with running untrusted code that could potentially harm systems or compromise data.

The platform’s support for multiple programming languages and rapid environment deployment capabilities make it a valuable asset for enterprises looking to harness AI technologies effectively. E2B’s open-source approach and deployment flexibility further enhance its appeal to a wide range of customers.

Perfect timing as Microsoft layoffs signal shift toward AI worker replacement

The funding round for E2B aligns with a significant shift towards AI agent technology, as evidenced by Microsoft’s recent layoffs coupled with increased reliance on AI agents for various tasks. However, infrastructure limitations have hindered widespread adoption of AI agents, a challenge that E2B aims to overcome with its specialized platform.

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The market potential for AI agents is substantial, with enterprises looking to automate a significant portion of manual tasks using AI technologies. E2B’s platform offers a solution that caters specifically to the needs of AI agents, positioning it as a key player in the evolving landscape of enterprise AI.

Open-source strategy creates defensive moat against tech giants like Amazon and Google

E2B’s open-source approach and focus on AI-specific use cases give it a competitive edge against industry giants like Amazon, Google, and Microsoft. The company’s commitment to creating an open standard for AI agent interactions with computing resources has garnered widespread adoption and positioned E2B as a leader in the field.

The platform’s unique capabilities, such as quick sandbox creation and enterprise-grade features, make it an attractive choice for companies seeking secure and scalable AI infrastructure. E2B’s network effect and open-source sandbox protocol further solidify its position in the market.

Enterprise features like 24-hour sessions and 20,000 concurrent sandboxes drive Fortune 100 adoption

E2B’s success in the enterprise sector can be attributed to its tailored features for large-scale AI deployments. The platform’s ability to scale and provide essential security and compliance features caters to the needs of Fortune 100 companies, making it a preferred choice for AI infrastructure.

Advanced capabilities like comprehensive logging, monitoring, and network security controls set E2B apart in the market, ensuring that enterprises have the necessary tools to deploy AI agents safely and effectively. The platform’s flexibility and granular controls further enhance its appeal to a diverse range of customers.

Insight Partners’ $21M bet validates AI infrastructure as next major software category

Insight Partners’ investment in E2B signifies a growing confidence in AI infrastructure companies and their potential impact on the industry. The significant funding will support E2B’s expansion efforts, including enhancing platform features and supporting its growing customer base.

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The investment will enable E2B to strengthen its position as a leader in AI infrastructure, providing secure and scalable solutions for enterprises looking to leverage AI technologies. Insight Partners’ backing further validates E2B’s vision and potential to shape the future of AI deployment.

The infrastructure play that could define enterprise AI’s next chapter

E2B’s rapid growth and success underscore a significant shift in how enterprises approach AI deployment. As AI agents become increasingly integral to business operations, the need for specialized infrastructure like E2B’s platform becomes paramount.

The emergence of E2B as essential infrastructure highlights the evolving landscape of enterprise AI, where security, compliance, and scalability play crucial roles in determining the success of AI initiatives. As AI agents continue to handle a growing share of tasks, platforms like E2B will be instrumental in ensuring their safe and efficient operation.

For enterprise leaders navigating the AI landscape, investing in specialized infrastructure like E2B may prove to be the key to unlocking the full potential of AI technologies in the modern business environment.

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