The 53-year-old and 31-year-old appeared in Melbourne Magistrates Court on Friday morning, facing charges related to alleged offenses in Syria.
They arrived in Melbourne on Thursday evening, part of a larger group of women and children from a Syrian refugee camp.
In a busy courtroom, the women were presented separately.
The older woman, wearing a pink hijab, appeared first, followed by the younger woman in a black hijab.
Both women have been remanded in custody until Monday, when they will seek bail.
Authorities claim that the 53-year-old traveled to the area with her family in 2014 and was involved in purchasing a female slave for $10,000, keeping her in her home knowingly.
The younger woman is also accused of knowingly harboring a female slave in her Syrian residence in 2014.
Police report that Kurdish forces detained the pair in 2019, and they were held with family members in the Al Roj Internally Displaced Persons camp.
Another woman, who arrived in Sydney on Thursday evening, is one of three women charged after a lengthy investigation that began when they traveled with partners intending to join Islamic State.
She is scheduled to appear at a NSW Bail Division court via video link on Friday.
The 32-year-old faces charges of entering a prohibited area and being part of a terrorist organization.
Her lawyers are seeking an urgent psychologist report to support her release bid, as indicated by the Commonwealth Director of Public Prosecutions.
AFP Assistant Commissioner Counter Terrorism Stephen Nutt noted that planning for the return of individuals from the Middle East began in 2015, with the investigation still ongoing.
A senior AFP officer declined to comment on Thursday regarding the welfare of the children, many born in Middle Eastern prison camps known for harsh conditions and extremist groups.
These children are expected to need substantial support to adapt to life in Australia and to assess any potential radicalization experienced while abroad.
Some women reportedly traveled willingly to support partners fighting for Islamic State, while advocates claim others were coerced or went to keep their families together.

